Pmdtm18-t simple vector kit
WebpMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers … WebThe pGEM®-T Vector size is 3000bp and the pGEM®-T Easy Vector size is 3,015bp. 6 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll …
Pmdtm18-t simple vector kit
Did you know?
WebpMD 18-T Vector是一种高效克隆PCR产物 (TA Cloning) 的专用载体。 本载体由pUC18载体改建而成，在pUC18载体的多克隆位点处的 Xba I 和 Sal I 识别位点之间插入了 Eco R V 识别 … WebFull Sequences from Addgene (1) Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger …
Web2. 利用Taq酶能够在PCR产物的3’末端加上一个非模板依赖的A，而T载体是一种带有3’T突出端的载体，在连接酶作用下，可以把PCR产物插入到质粒载体的多克隆位点，可用于PCR产物的克隆和测序。 主要试剂. 1. pMDTM18-T Simple Vector Kit(Takara公司) 2. TAE电泳缓冲液 3. WebNov 19, 2024 · T-Vector pMD19 (Simple) (Search Vector Database) Backbone manufacturer Clontech Backbone size (bp) 6332 Modifications to backbone T-Vector pMD19 (Simple) was digested with EcoR V and inserts were ligated into the backbone. Vector type Plant Expression Promoter 35s core promoter Tag / Fusion Protein GFP derived from pMDC107 …
Web【试剂与器材】 （一） 试剂 1. pMDTM18-T Simple Vector Kit（Takara 公司）。 2. TAE 电泳缓冲液 3.琼脂糖（Agarose） 4.6×电泳加样缓冲液：0.25％ 溴粉蓝，40％(w/v) 蔗糖水 … WebT-Vector pMD™19 (Simple) 1 ug. USD $106.00. T-Vector pMD19 (Simple) is a linearized vector with a single 3'-terminal thymidine at both ends. The T-overhang ends at the …
Web2 GAL4 Two-Hybrid Phagemid Vector Kits Vectors ♦ The pAD-GAL4-2.1 phagemid vector undigested (20 μg) ♦ The pBD-GAL4 Cam phagemid vector undigested (20 μg) Store the pAD-GAL4-2.1 and pBD-GAL4 Cam phagemid vectors at –20°C. Control Plasmids ♦ The pGAL4, pBD-WT, pAD-WT, pBD-MUT, pAD-MUT, and pLamin C control plasmids for the …
WebSep 6, 2024 · Hi Guys.. I hope you can help me. I successfully cloned 426 Bp fragment into pMD-18 T simple vector confirmed my sequencing. Remember pMD18 is a TA cloning vector. ... They have used the kit DCFDA ... tow truck dandenongWebpmd18-t simple vector 图谱序列. 1201. 1251. 1301. 1351. 1401. 1451. 1501. 1551 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg … tow truck dartmouthWebPmd18 T Simple Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. 86 stars, based on 1 article reviews. Price from $9.99 to $1999.99. tow truck damage to my carWebpMD20 and pMD19 (Simple) T-Vectors are linearized PCR cloning vectors (cleaved by EcoRV) with single 3’-terminal thymidine residues (dT) at both ends. These T-overhangs at the cloning site improve the efficiency of ligation … tow truck davenportWebThe vector kit has four components 1. This handout, with preliminary description of the new vectors 2. The microtiter vector array, containing vectors described in this handout. Contained in this package, store -20°C. Instructions for handling the samples are described on the microtiter wills and on p.20 of this handout 3. tow truck dashboardWebSIMPLE CARPORT 10 x 20 x 6; QUICK COVER 14 x 21 x 5; THREE SIDED 14 x 25 x 8; GARAGE 20 x 25 x 9; DURABLE GARAGE 18 x 20 x 10; OUR ADVANTAGES Buy Factory Direct Quick … tow truck damaged my carWeb（二）连接反应（以 Takara 公司 pMDTM18-T Simple Vector Kit 为例） 1）在微量离心管中配制下列 DNA 溶液，全量为 5 μl。 pMDTM18-T Simple Vector 1 μl. Insert DNA 0.1 pmol～0.3 pmol. dH2O up to 5 μl. 2）加入 5 μl（等量）的 Solution I。 3）16℃反应 30 分钟 … tow truck davis ca