WebFeb 28, 2024 · Arabidopsis thaliana var. Col-0 wildtype plants and transgenic lines were grown on ... Carlsbad, U.S.A.) into the Gateway site of the vector pHGWFS7.0 101 … WebApr 1, 2007 · Mature anthers were fixed in 4% paraformaldehyde and 0.25% glutaraldehyde under vacuum. Fixed samples were then dehydrated through a graded ethanol series followed by a t-butanol series, ... The binary vector pHGWFS7 was modified for the GUS gene under the control of a 2000 ...
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WebAug 1, 2010 · GATEWAY-compatible destination vectors ( K arimi et al . 2002 ) for promoter analysis (pHGWFS7) and overexpression (pH7FWG2.0, pK7WG2D.1) were ordered from the Department of Plant Systems Biology, VIB-Ghent University (Ghent, Belgium). The vector pAVA319 contained a translation leader (TL) and was obtained from ABRC. WebThe destination vector (pHGWFS7.0) used here is efficient for promoter expression analysis (Karimi et al. 2002). Site specific recombination properties of Gateway system allowed recombination of the Adh promoter from the pENTR_Adh to … san bernardino auction cars
A chloroplast envelope membrane protein containing a putative …
WebFeb 28, 2024 · In cold-primed plants, the pleiotropically stress regulated genes CHS (chalcone synthase; At5g13930) and PAL1 (phenylalanine ammonium lyase; At2g37040) were stronger activated by a second cold... WebOct 20, 2016 · The vector pH35 was constructed from Gateway promoter-reporter vector pHGWFS7, which contained a GFP-GUS fusion for plant expression, hygromycin resistance gene ( HygR) for plant transformation and spectinomycin/streptomycin resistance (Sm/SpR) for bacterial transformation (Karami et al. 2009 ). WebThis intermediate entry vector was recombined with the pHGWFS7 vector by using LR clonase. The resulting construct was transferred to Arabidopsis (Col-0) by Agrobacterium-mediated transformation. Primary transformants were selected on Gamborg's B5 plates containing hygromycin (50 µg/liter). san bernardino auditor controller\\u0027s office