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Phgwfs7.0

WebFeb 28, 2024 · Arabidopsis thaliana var. Col-0 wildtype plants and transgenic lines were grown on ... Carlsbad, U.S.A.) into the Gateway site of the vector pHGWFS7.0 101 … WebApr 1, 2007 · Mature anthers were fixed in 4% paraformaldehyde and 0.25% glutaraldehyde under vacuum. Fixed samples were then dehydrated through a graded ethanol series followed by a t-butanol series, ... The binary vector pHGWFS7 was modified for the GUS gene under the control of a 2000 ...

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WebAug 1, 2010 · GATEWAY-compatible destination vectors ( K arimi et al . 2002 ) for promoter analysis (pHGWFS7) and overexpression (pH7FWG2.0, pK7WG2D.1) were ordered from the Department of Plant Systems Biology, VIB-Ghent University (Ghent, Belgium). The vector pAVA319 contained a translation leader (TL) and was obtained from ABRC. WebThe destination vector (pHGWFS7.0) used here is efficient for promoter expression analysis (Karimi et al. 2002). Site specific recombination properties of Gateway system allowed recombination of the Adh promoter from the pENTR_Adh to … san bernardino auction cars https://changingurhealth.com

A chloroplast envelope membrane protein containing a putative …

WebFeb 28, 2024 · In cold-primed plants, the pleiotropically stress regulated genes CHS (chalcone synthase; At5g13930) and PAL1 (phenylalanine ammonium lyase; At2g37040) were stronger activated by a second cold... WebOct 20, 2016 · The vector pH35 was constructed from Gateway promoter-reporter vector pHGWFS7, which contained a GFP-GUS fusion for plant expression, hygromycin resistance gene ( HygR) for plant transformation and spectinomycin/streptomycin resistance (Sm/SpR) for bacterial transformation (Karami et al. 2009 ). WebThis intermediate entry vector was recombined with the pHGWFS7 vector by using LR clonase. The resulting construct was transferred to Arabidopsis (Col-0) by Agrobacterium-mediated transformation. Primary transformants were selected on Gamborg's B5 plates containing hygromycin (50 µg/liter). san bernardino auditor controller\\u0027s office

The Plant Journal 64 The plastidial glucose-6 …

Category:Cold-priming of chloroplast ROS signalling is …

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Phgwfs7.0

Cold-priming of chloroplast ROS signalling is developmentally

WebSep 14, 2011 · GATEWAY ™-compatible destination vectors (Karimi et al., 2002) for protein subcellular localization (pH7FWG2,0), promoter analysis (pHGWFS7,0), overexpression (pH7WG2D,1) and hairpin RNA interference (pH7GWIWG2(I),0) were purchased from the Functional Genomics Division, VIB-Ghent University (Ghent, Belgium). The LR reaction was … WebLOG IN. Minimum purchase order is 150,00€. Free shipping on all products. Leave this field blank. Gateway vectors

Phgwfs7.0

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WebJul 23, 2010 · To assess the suitability of the NAPA promoter we made a translational fusion between the promoter fragment and the eGFP (encoding enhanced green fluorescent protein) and uidA [encoding β-glucuronidase (GUS)] genes present in the binary vector pHGWFS7.0 (Karimi et al., 2002), and stably expressed this fusion in Arabidopsis plants. … Webwere sterilized with 0.3% Sodium Hypochlorite and pre-germinated on 0.9% bactoagar plates at 30 C. Seven day old seedlings were transferred to sterilized sand and grown for two weeks without fertilizer. Plantlets were then transferred to liquid ½ Hoagland containing 25 M phosphate for another week before treating them either with GSE,

WebVIB-UGent Center for Plant Systems Biology. Gateway Vectors. Collection. pKGWFS7. Web... pHGWFS7 for promoter-gus constructs ( Fig. 1) : This vector with binary pPZP200 backbone ( Hajdukiewicz et al. 1994) contains CmR-ccdB selection flanked by attR1 and …

WebOct 1, 2014 · A sequence of approximately 2kb preceding the translation initiation site of the OsAPx1 gene was isolated, cloned into pENTR vector and recombined in pHGWFS7 vector, whichallows the fusion of the promoter sequence with two report genes, Gfp and Gus, and confers resistence to hygromycin.The construction was named pPROM1. The … WebJan 5, 2024 · Finally, MtCIR1 recombined with pHGWFS7.0 (Supplemental Fig. S3). After sequence confirmation, the terminal construct p MtCIR1 -GUS was transformed into …

WebGATEWAY -compatible destination vectors (Karimi et al., 2002) for protein subcellular localization (pH7FWG2,0), pro- moter analysis (pHGWFS7,0), overexpression (pH7WG2D,1) and hairpin RNA interference (pH7GWIWG2(I),0) were purchased from the Functional Genomics Division, VIB-Ghent University (Ghent, Belgium).

WebNWCG san bernardino bail scheduleWebAug 1, 2010 · GATEWAY-compatible destination vectors (Karimi et al. 2002) for promoter analysis (pHGWFS7) and overexpression (pH7FWG2.0, pK7WG2D.1) were ordered from … san bernardino auto accident lawyerWebMar 24, 2024 · We prepared genomic fragments (1) from 4.2 kbp upstream of the translation start site of HAM1 (AT2G45160), which contains the promoter region, to the TS of miR171a; (2) from the TS to the 3′-UTR end; and (3) from the linearized vector (from pHGWFS7.0) by PCR using primer nos. 7 and 8, 9 and 10, and 11 and 12, respectively (see Supplemental ... san bernardino bad credit auto dealersWebwith the pHGWFS7 vector by using LR clonase. The resulting construct was transferred to Arabidopsis (Col-0) by Agrobacterium-mediated transformation. Primary transformants … san bernardino barstow courtWebantiporter genes into the GATEWAYTM promoter cloning vector pHGWFS7. The Pokkali promoter expressed the β-glucuronidase or GUS gene ~25-fold more efficiently than the CaMV 35S promoter in rice calli, while that of IR64 was 7-fold ... using PCR mix containing PCR reaction buffer (Invitrogen), 1.33 mM MgCl2, 0.1 mM dNTP mix, 2.66% DMSO, 0.5U … san bernardino backflow certificationWebFeb 1, 2016 · genomic DNA and the binary vector pHGWFS7.0_RD29A (positive control) were completely digested with BamH1 and the GUS gene was used as probe. Transgenic lines (P-1 and P-2) s howed one band. san bernardino barber shop fight owner killedWebCCATTAACAAATCTCCG-30; and a T-DNA insertion primer, LBb1.3, 5 0-ATTTTGCCGATTTCGGAAC-3 ). The zfp5 gis3, ... RNAi construct, whereas pHGWFS7 was used for the pGIS3:GUS construct. For all cloning constructs, targeted gene-specific fragments were first PCR-amplified from cDNA san bernardino bar association website