Maleimide activated bsa
WebMaleimide Activated BSA used as a carrier protein for haptens to enable the immune response. Catalog #: M1316 abID: Buy from Abcam Datasheet Bulk Orders? Share … WebMaleimide Activated BSA is lyophilized in a buffered solution containing 0.1M sodium phosphate, 0.15M sodium chloride, 0.1M EDTA, with stabilizer; pH 7.2. 77125 Imject …
Maleimide activated bsa
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WebThis is a replacement product for CM52103. CellMosaic’s maleimide activated b ovine serum albumin (also known as BSA or "Fraction V") HL is designed for direct conjugation … Web4 jul. 2008 · As positive controls for the vaccination regimen, mice were vaccinated with bovine serum albumin (BSA) or keyhole limpet hemocyanin (KLH). We also conjugated GU81 via a cysteine residue to maleimide-activated KLH or BSA carrier proteins and vaccinated mice with this construct.
WebThis product is manufactured by Expedeon Inc. and distributed by Novus Biologicals. Background Maledimide and periodate activated labels are designed to facilitate the …
WebThe Thermo Scientific Imject Maleimide-Activated BSA Spin Kit contains stabilized, lyophilized Maleimide-Activated BSA (in PBS with stabilizer) and accessory … http://genetics.wustl.edu/tslab/protocols/protein-stuff/bsa-conjugation-with-maleimide-pierce/
WebActive HOOK ™ BSA is maleimide activated BSA, by the addition of sulfoSMCC cross-linker. The Active HOOK ™ BSA rapidly couples to free sulfhydryl groups on peptides …
WebMaleimide Activated BSA is provided in lyophilized, ready to-use form, eliminating the need for time-consuming activation and purification steps of the carrier protein. Maleimide Activated BSA contains approximately 5-15 maleimide groups for each BSA … grease solubilityWeb31 aug. 2012 · The polystyrene surface is pre-coated with maleimide-activated BSA or KLH, and peptides are added directly to the wells of microtitre plates for subsequent coupling. The efficiency of DCLM was tested with four different peptides (TvExt-1, TvExt-2, TvCt-1, or TvCt-2) and subsequent standard indirect ELISA with polyclonal antibodies … choose all of the true statements belowWebBovine Serum Albumin (BSA) is chemically modified on a single cysteine residue with Azide-PEG3-Maleimide (MW 369.37). Provided as lyophilized solid. To prepare a … choose a letter a-zWebwww.ncbi.nlm.nih.gov choose a lewis structure for gebr3-WebImject Maleimide Activated BSA Kit (Pierce) Includes: Imject Maleimide Activated BSA (AcBSA) 5 x 2 mg All Conjugation and Purification Buffers Prepacked Gel Filtration Columns 5 x 5 ml See the instructions from the Pierce kit. A. Conjugation Procedure 1. Reconstitute one vial of the maleimide-activated protein by adding 200 ul of ultrapure ... grease solvent hand cleanerWeb1 okt. 2024 · Maleimide Lysine Conjugation Maleimides are a type of Michael acceptor and therefore undergo addition reactions with thiols. For this reason, maleimides are most often used as reagents for cysteine bioconjugation in literature. Between pHs of 6.5 and 7.5, they are highly selective for thiol groups. choose all parts of the cell theoryWeb29 jul. 2024 · For example, m is about 15 maleimide groups per protein in maleimide activated BSA commercially available from Sigma-Aldrich of St. Louis, Mo.; m is about 80 maleimide groups per protein in maleimide activated KLH commercially available from Sigma-Aldrich; m is in a range of about 15 to about 25 maleimide groups per protein in … choose all function of trichomes