Klf5 chip
WebNov 9, 2024 · Klf5 is essential for bi-potential cell fate; a single Klf5 -overexpressing embryonic stem cell (ESC) generates terminally differentiated embryonic and extra … WebIn epigenetic and transcriptional analyses of ESCC cell lines, we found the transcription factors TP63, SOX2, and KLF5 to be part of a core regulatory network that determines chromatin accessibility, epigenetic modifications, and gene expression patterns in these cells. A combination of epigenetic i …
Klf5 chip
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WebOct 7, 2024 · To determine direct gene targets of KLF5, we defined the cistrome of KLF5 using ChIP-seq in both Calu-3 and 16HBE14o- lung epithelial cells lines. Occupancy site concordance analysis revealed that KLF5 co-localized with the active histone modification H3K27ac and also with binding sites for CCAAT Enhancer Binding Protein Beta (C/EBPβ). WebFeb 20, 2024 · ChIP-seq data Rogerson et al., 2024 demonstrate that KLF5 strongly binds to the JUP and CCNE1 enhancers but low levels are observed at the MYBL2 enhancer . KLF5 depletion ( Figure 3—figure supplement 1H ) caused reduced expression of all three target genes ( Figure 3H ).
WebIndeed, Klf5-null mice show developmental defects at blastocyst stage, due to the defective establishment of the inner cell mass. Moreover, Klf5 knockdown in ESCs results in the … WebSep 3, 2024 · This new KLF5 target gene programme has potential prognostic significance as high levels correlate with poorer patient survival. Thus, the repurposing of KLF5 for …
WebAntibodies that detect KLF5 can be used in several scientific applications, including Western Blot, Immunocytochemistry, Immunohistochemistry, ChIP and ELISA. These antibodies target KLF5 in Human, Mouse and Rat samples. Our KLF5 polyclonal, monoclonal, recombinant monoclonal and recombinant polyclonal antibodies are developed in Rabbit … WebKLF5 was bound primarily at promoters, intergenic regions, and within genes. To determine which of the genes that were differentially expressed on KLF5 depletion were direct targets, we intersected the Calu-3 ChIP-seq dataset with the DEGs identified by RNA-seq ( Fig. 2 B ).
WebMar 17, 2024 · ChIP with the KLF5 antibody from R&D (#AF3758) was performed using the SimpleChIP® Enzymatic Chromatin IP Kit (Cell Signaling, #9003) following the manufacture’s instructions. Primers for these analyses are listed in Supplementary Table 3. The SYBR green (Takara, Mountain View, CA) method was used with the 7500 Fast Real …
WebTo investigate whether KLF5 acetylation affects the binding of KLF5 to KBE and TIE of MYC promoter, we conducted a ChIP assay in HepG2 cells transfected with pcDNA3-FLAG … michael rorls marylandWebOct 1, 2024 · TP63, SOX2, and KLF5 Establish a Core Regulatory Circuitry That Controls Epigenetic and Transcription Patterns in Esophageal Squamous Cell Carcinoma Cell Lines - ScienceDirect Gastroenterology Volume 159, Issue 4, October 2024, Pages 1311-1327.e19 Original Research Full Report: Basic and Translational—Alimentary Tract michael rosans bathroom fiascoWebNov 9, 2024 · Although Klf5 has the ability to induce ICM and TE genes based on our ChIP-seq data, Klf5 mediates gene transcription in a cell-type- and context-specific manner. In pluripotent ESCs, Klf5 overexpression induced multiple pluripotency transcription factors, including Nanog, ... michael rosamond baseballWebJun 11, 2024 · For instance, ChIP-sequencing showed that KLF5 binding sites in C2C12 myotubes and 3T3-L1 adipocytes differ, and only a small fraction of sites are common between the two cell types (Figure 4A). Moreover, even in the same C2C12 cells, differentiation alters KLF5 bindings sites (Figure 4B) . Likewise, KLFs may control … michael rosbach bonnWebWe performed RNA sequencing, immunohistochemistry, western blotting, real time-PCR, ELISA, luciferase assay, chromatin immunoprecipitation (ChIP), and flow cytometry to demonstrate the effects of KLF5 on CD8 + T cell infiltration and related molecular mechanism. Single-cell RNA sequencing and spatial transcriptomics analysis were … michael rosbash brandeisWebOct 28, 2024 · Klf5 is an essential regulator of cell fate during early embryonic development, and it can also be used to generate pluripotent stem cells and to maintain their potency and self-renewal... michael rosas carthage nyWebMar 17, 2024 · ChIP with the KLF5 antibody from R&D (#AF3758) was performed using the SimpleChIP® Enzymatic Chromatin IP Kit (Cell Signaling, #9003) following the … how to change schwab password