NettetFollowed by 3x washes with PBS with 0.3% Triton X-100 for 5 min each, nuclei were labeled with Hoechst 33342 (Thermo Fisher Scientific) for 5 min and then were mounted with Fluoromount-G (SouthernBiotech, Birmingham, AL). Phospho-SMAD, ID3 and BMP6 immunostaining was performed on floating sections. Nettet10. apr. 2024 · Cells were washed twice with PBS (−) and then incubated with secondary antibodies Alexa Fluor 488, Alexa Fluor 555, or Alexa Fluor 647 (1:250; Thermo Fisher Scientific) and Hoechst 33258 (0.5 μg/mL; Sigma-Aldrich) for 1 h at room temperature.

NucBlue™ Live ReadyProbes™ Reagent (Hoechst 33342)

NettetPA3-910 detects Grasp65 from human, mouse, and rat samples. PA3-910 has been successfully used in Western blot, IHC (paraffin), immunofluorescence, immunocytochemistry and immunoprecipitation procedures. The PA3-910 immunogen is a GST fustion protein containing the C-terminal residues (202-447) of rat Grasp65. 靶标信息 NettetThermo Scientific Pierce Hoechst 33342 Fluorescent Stain is a high-quality solution of Hoechst dye for fixed- and live-cell fluorescent staining of DNA and nuclei in cellular … Thermo Scientific Pierce Hoechst 33342 Fluorescent Stain is a high-quality … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes Hoechst 33342 SYTO 9 Green Nucleic Acid Stain SYTO 82 Orange Nucleic Acid … The Thermo Scientific FITC Antibody Labeling Kit is a multi-component kit … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes is friendster still active

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NettetThermo Scientific™ Hoechst 33342 Solution (20 mM) High-quality Hoechst dye solution for fluorescent staining of DNA and nuclei in fixed- or live-cell imaging techniques. … NettetCombining Hoechst and an EdU incorporation assay with Myc protein staining, we evaluated Myc protein distribution throughout the cell cycle and observed Myc variability to be independent of cell-cycle phases (Figure S2 A). Myc variability was also independent of cell-cycle progression within G1 ( Figure S2 B) and local cell density ( Figure S2C). NettetThermo Scientific™ Hoechst 33342 Solution (20 mM) High-quality Hoechst dye solution for fluorescent staining of DNA and nuclei in fixed- or live-cell imaging techniques. Brand: Thermo Scientific™ 62249 Additional Details : Weight : 0.10000kg View more versions of this product Product Code. 10150888 125.00 EUR / 5mL Estimated Shipment: 14-04-2024 is friendship overrated