2024 Hcrt cre

Hcrt cre

Author: owyf

August undefined, 2024

http://www.gensat.org/TRAP_listing.jsp WebNov 19, 2024 · The retro-tracing virus was restrictedly injected into the bilateral VTA in Hcrt-Cre mice (Fig. 1 e and f). Half of the virus-injected mice were then subjected to CUMS for 21 days, and the others were housed under SPF conditions for control. All CUMS and control groups were simultaneously killed for immunofluorescence staining.

GENSAT Brain Atlas of Gene Expression in EGFP, tdTomato, and Cre ...

WebImages for hypocretin ( Hcrt ) TRAP (translating ribosome affinity purification) founder line JD218 This line is available from The Jackson Laboratory, stock ID 028619 Click on a thumbnail of mouse brain to examine the image in detail. WebJun 9, 2024 · Cre-recombinase (Hcrt-Cre) (82) selectively in hypocretin neurons under the control of a specific hypocretin promoter were used in t his study. Mice were group housed and maintained on a 12-12 hr cover letter examples for warehouse job

028619 - Hcrt bacTRAP Strain Details - The Jackson …

WebNov 27, 2024 · Hcrt deficient mice lacking one copy of the 5HTT gene ... DR neurons of mice harboring loxP site-flanked alleles of both HCRT receptors 1&2 genes 27 with AAV … WebCre mice grouped by gene. (291 Cre lines of which 246 have images.) In collaboration with the Intramural Program of the National Institute of Mental Health (PI: Dr. Charles Gerfen), GENSAT offers transgenic BAC-Cre recombinase driver lines that allow for cell-specific gene manipulations in the central nervous system of the mouse. Web7z¼¯' êQ "fz H±¨BSDIFF40Z= Á _Òs BZh91AY&SYJ°è¥\×ÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿà2 {¸ CB Ø{ÞU÷Ûï¥Û ... cover letter examples for writers

The evolutionarily conserved miRNA-137 targets the neuropeptide

WebHere we used a new Hcrt-IRES-Cre mouse line that achieved ChR2 expression eutopically in Hcrt neurons by the endogenous Hcrt gene locus, combined with the powerful Cre … WebRibosome-bound mRNAs from Hcrt -expressing neurons of the hypothalamus can be purified using EGFP as an affinity tag. If you need help with your order, contact … brickell food and wine festivalWebW-Tg(HCRT-cre,-EGFP)B5Ahky; NBRP Rat No: 0778: Type: transgenic: Source: National BioResource Project for the Rat in Japan: Origin: This transgenic rat strain was established by Dr. Yamanaka (Nagoya University) in 2012. Background strain: Wistar rat (CLEA Japan). plasmid backbone: pCR2.1. Transgene: human HCRT gene promoter, cre recombinase ... brickell food and wine

"WebHcrt: hypocretin: JD218: 028619: RRID:IMSR_JAX:028619: Expression of mouse gene hypocretin ( Hcrt ) is observed in neurons in the lateral hypothalamic area (LHA) of the mouse brain. Hcrt codes for a precursor protein that will give rise to the orexin A and orexin B peptides: Htr3a: 5-hydroxytryptamine (serotonin) receptor 3A: GM443: 030900 ... " - Hcrt cre

Hcrt cre

JCI - Impaired hypocretin/orexin system alters responses to …

WebMay 1, 2024 · Using a Hcrt-Cre transgenic mouse line combined with optogenetic release of Hcrt in a temporal manner, we have shown antagonism of HcrtR2 mainly mediates the … WebJul 23, 2024 · Hcrt-LH and LepRb-LH neurons: physiology and behavior. To establish selective Hcrt-LH targeting, we developed a knock-in IRES-Cre mouse line (Hcrt-Cre; Supplementary Fig. 1a,b).Following intra-LH ...

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WebJun 9, 2024 · C57BL/6J mice (4–6 months old) expressing GFP (Hcrt-GFP) (18, 38, 39, 44) or Cre-recombinase (Hcrt-Cre) selectively in hypocretin neurons under the control of a specific hypocretin promoter were used in this study. Mice were group housed and maintained on a 12-hour light/12-hour dark cycle (lights on at 6:00 am) with free access … WebTo investigate the hypothesis that miR-137 downregulation will cause an increase in Hcrt, we injected viral vectors containing a sponge sequence of these miRNAs into the lateral …

http://www.informatics.jax.org/recombinase/summary?driver=HCRT

WebG@ Bð% Áÿ ÿ ü€ H FFmpeg Service01w ... WebAug 2, 2024 · Hcrt-Cre transgenic mice and C57BL/6J mice were used to purify HCRT and MCH cells, respectively. In this method, dissociated hypothalamic cells are dispensed in …

WebHere we used a new Hcrt-IRES-Cre mouse line that achieved ChR2 expression eutopically in Hcrt neurons by the endogenous Hcrt gene locus, combined with the powerful Cre-dependent EF1α promoter. This combination produces many more ChR2 molecules per neuron than the original 1.2 Kb mouse promoter packaged into lentiviral vectors . …

http://www.informatics.jax.org/recombinase/specificity?id=MGI:3839422&system=head brickell food tourWebJan 1, 2024 · Methods. Hcrt cre rats were generated using a CRISPR/Cas9-based approach. Viruses encoding optogenetic probes were injected into the perifornical lateral hypothalamic (PeFLH) area, optogenetic fibres were embedded in the PeFLH, BF, or LC, and changes in anaesthesia state under 1.4 vol% or 0.8 vol% isoflurane were determined. cover letter examples for research positionWebMethods: Hcrt cre rats were generated using a CRISPR/Cas9-based approach. Viruses encoding optogenetic probes were injected into the perifornical lateral hypothalamic … cover letter examples hospitality managementhttp://gensat.org/cre.jsp cover letter example shortWebJun 28, 2024 · Taking the LHA-BF neural circuit in Hcrt-Cre mice as an example, the opsin is typically introduced to the LHA neurons by injecting a virus containing ChR2 (A) or NpHR (B). After 3 weeks to allow for expression of the opsin, LHA-BF axon terminals can be targeted with corresponding light pulses from the optical fiber to excite ( A ) or inhibit ... cover letter examples healthcare assistanthttp://www.gensat.org/TRAPFounderView.jsp?founder_id=93983&gene_id=36 cover letter examples grocery storeWebTg (HCRT-cre)1Stak. Mutation details : A 3.2 kb fragment of the 5' upstream region of the human HCRT gene drives expression of cre and eGFP. The eGFP gene is separated … brickell food places