WebRecombinant CHO TF70R viable cell density (A), percentage of cell viability (B), rh-tPA production (C), cell-specific rh-tPA production (D), glucose consumption (E), lactate production (F), and glutamine consumption (G) of cell cultures at 37°C (closed squares), and under temperature downshift (TDS) to 30°C at 48 h (open circles). Dotted line denotes … WebJul 1, 2001 · We then tried to use the same trained model to simulate the behavior of recombinant CHO TF70R. This was achieved, but necessitated to synchronize the time-scales of the two cell lines to compensate for their different specific growth rates. Keywords: animal cells, artificial neural network, CHO, growth kinetics, modelization.

A general artificial neural network for the modelization of …

WebRecombinant CHO TF70R cells are able to grow and produce t-PA on serum-free medium BIOPRO1 (BioWhitaker Europe, Belgium). The purpose of the present study was to determine the effect of medium... Web37 C. CHO Tf70R cells producing human tissue-like plasminogen activator (t-PA) were kindly provided by Pharmacia & Upjohn, Sweden. This recombinant cell line contained … partsclick

Kinetics of cell growth and rh-tPA production.

http://www.bioline.org.br/pdf?ej06008 WebRecombinant CHO cells were grown in stirred batch suspension cultures at 37°C; CHO 320, producing human was grown in RPMI 1640 & 5% FCS & 1 µM methotrexate (MTX); CHO tF70R, producinghuman tissue plasminogen activator (t-PA), was grown in a proprietary serum (Biopro 1,free medium BioWhittaker, Belgium). Both constructs use the SV40 … CHO cell cultures We used two cell strains: CHO K1 (CCL 61, ATCC) and CHO TF70R: t-PA producing recombinant CHO, also capable of growth in suspension, and supplied to us by Dr. T. Björing (Pharmacia-Upjohn). The CHO K1 were grown in Bellco spinner flasks and the CHO TF70R in Techne spinner flasks (500 ml total volume, 250 ml of medium) with ... オリックス銀行 定期預金 7年